UE-D4054◆DAPI和Hoechst 染料非常相似，该如何选用？DAPI是一种非常普通的蓝色核复染荧光染料，能对固定和通透的细胞和组织的细胞核进行非常明亮的标记。遗憾的是，人们普遍认为它是介于半通透性到非通透性之间的染色剂，对活细胞的染色效果也不一致。Hoechst 33342染料是细胞通透性染料，与DAPI染色有相似的染色结合机制和荧光颜色；它是活细胞成像的首选，且对固定细胞的标记效果和DAPI一样好。◆DAPI染色的工作液浓度一般为多少，要怎么稀释？该试剂用于细胞染色时，需要根据具体的实验目的确定工作液浓度，常用的浓度0.5-10μg/ml，直接用水稀释即可。◆在细胞膜和细胞核双染实验中，细胞膜染色用DiI，细胞核染色用DAPI，细胞固定后，先染色DiI还是先染色DAPI？先用DiI对细胞膜进行染色，后用DAPI染核。◆DAPI和Hoechst可以用来示踪细胞吗？不可以，这些染料与DNA和RNA的结合会影响核酸的正常功能，扰乱转录和增殖。◆DAPI能否用来染色植物细胞的原生质体？可以，有文献报道使用DAPI染色原生质体，但是原生质体比较脆弱，DAPI对原生质体的杀伤力可能比较大。◆该试剂可对酵母菌进行荧光标记吗？有文献用DAPI染酵母样品，但我们目前没有相关经验，建议查看相关文献资料进行参考。◆该试剂可以染活细胞吗？DAPI染色时，细胞均需要固定操作，对于活细胞的染色效果较差。◆请问可以用于石蜡切片的免疫组化，进行染核吗？可以的。
4.A Network Pharmacology Approach to Investigate the Anticancer Mechanism and Potential Active Ingredients of Rheum palmatum L. Against Lung Cancer via Induction of ApoptosisQing Zhang, Jia Liu, Ruolan Li, Rong Zhao, Mengmeng Zhang, Shujun Wei, Dong Ran, Wei Jin, Chunjie WuFrontiers in Pharmacology
5.Antiepileptic Effects of Cicadae Periostracum on Mice and Its Antiapoptotic Effects in H2O2-Stimulated PC12 Cells via Regulation of PI3K/Akt/Nrf2 Signaling PathwaysQing Zhang,Ruo-Lan Li,Ting Tao,Jia-Yi Sun,Jia Liu,Ting Zhang,Wei Peng,Chun-Jie WuVolume20216.SARS-CoV-2 ORF10 suppresses the antiviral innate immune response by degrading MAVS through mitophagyXingyu Li,Peili Hou,Wenqing Ma,Xuefeng Wang,Hongmei Wang,Zhangping Yu,Huasong Chang,Tiecheng Wang,Song Jin,Xue Wang,Wenqi Wang,Yudong Zhao,Yong Zhao,Chunqing Xu,Xiaomei Ma,Yuwei Gao,Hongbin Hecellular & molecular immunology7.DHA alleviates diet-induced skeletal muscle fber remodeling via FTO/m6A/DDIT4/PGC1α signalingWei Chen,Yushi Chen,Ruifan Wu,Guanqun Guo,Youhua Liu,Botao Zeng,Xing Liao,Yizhen Wang,Xinxia WangBMC Biology8.Guanxin V alleviates acute myocardial infarction by restraining oxidative stress damage, apoptosis, and fibrosis through the TGF-β1 signalling pathwayBo Liang,Xiao-Xiao Zhang,Rui Li,Yong-Chun Zhu,Xiao-Jie Tian,Ning Gu Phytomedicine参考文献：
1.Assay for nanogram quantities of DNA in cellular homogenates
应用方向：DNA含量测定2.A simple cytochemical techniqUE for demonstration of DNA in cells infected with mycoplasmas and viruses应用方向：诊断细胞培养的支原体感染3.Stage-specific differences in cell cycle control in Trypanosoma brucei revealed by RNA interference of a mitotic cyclin
应用方向：流式细胞术中细胞核含量测定及染色体分选4.Loss of HSulf-1 up-regulates heparin-binding growth factor signaling in cancer 应用方向：评估细胞凋亡5.Direct measurement of femtogram amounts of DNA in cells and chloroplasts by quantitative microspectrofluorometry. 应用方向：免疫荧光法和原位杂交法检测细胞核和细胞器DNA6.SMAD2 and SMAD7 Involvement in the post-translational regulation of Muc4 via the transforming growth factor-β and interferon-γ pathways in rat mammary epithelial cells 应用方向：免疫荧光法和原位杂交法检测细胞核和细胞器DNA7.A simple cytochemical techniqUE for demonstration of DNA in cells infected with mycoplasmas and viruses 应用方向：取代溴化乙锭对琼脂糖凝胶中的DNA进行染色8.Comparison of ethidium bromide and 4", 6"-diamidino-2-phenylindole as quantitative fluorescent stains for DNA in agarose gels 应用方向：取代溴化乙锭对琼脂糖凝胶中的DNA进行染色9.Localization of polyphosphates in Saccharomyces fragilis, as revealed by 4´, 6-diamidino-2-phenylindole fluorescence 应用方向：DAPI可与多磷酸盐和其他聚阴离子，硫酸葡聚糖和SDS结合10.InflUEnce of S-adenosylmethionine on 4´, 6-diamidino-2-phenylindole dihydrochloride (DAPI)-induced fluorescence of polyphosphate in the yeast vacuole 应用方向：DAPI可与多磷酸盐和其他聚阴离子，硫酸葡聚糖和SDS结合11.Fluorescent Complexes of DNA With DAPI 4",6-diamidine-2-phenyl indole.2HCl or DCI 4",6-dicarboxyamide-2-phenyl Indole 应用方向：DAPI可与多磷酸盐和其他聚阴离子，硫酸葡聚糖和SDS结合
